Then, the goal proteins or subdomains of them containing the target lysine can be produced recombinantly and the methylation additionally examined at protein stage. The horizontal axis represents the H3 sequence and solicitor law Firms the vertical axis represents the amino acids by which the corresponding row is mutated. A) Discrimination factors of NSD1 for the popularity of amino acid residues at the positions subsequent to the goal lysine (K36 within the experiment proven here).

The enzymes concerned in lysine methylation have been first discovered to target histone and thus were initially named histone methyltransferases and histone demethylases, following the naming model for histone acetyltransferase and histone deacetylase. KMTs catalyze mono-, di-, or tri-methylation by transfering one, two, or three methyl teams, respectively, from S-adenosyl-L-methionine to the ε-amino group of a lysine residue.

For instance